Network analysis of maternal parenting practices and adolescent mental health problems: a longitudinal study.

BACKGROUND: An extensive literature has shown a strong connection between maternal parenting practices and adolescent mental health problems. However, it has been difficult for previous research to map a dynamic concurrent and prospective relationships within and between types of parenting practices and adolescent mental health problems. The present study addressed these issues using a network analysis approach and a longitudinal design. METHODS: This study involved 591 Chinese adolescents (249 males; mean age at T1 = 13.53) and their mothers (mean age at T1 = 39.71) at two time points (T1 and T2) with eighteen months apart. Mothers reported their parenting practices including warmth, monitoring, inductive reasoning, hostility, and harshness, while adolescents reported their mental health problems including anxiety, depression, aggression, and conduct problems. Network analysis was conducted for contemporaneous networks at T1 and T2 and temporal networks from T1 to T2. RESULTS: The contemporaneous networks revealed the negative association between monitoring and conduct problems served as the main pathway through which parenting practices and adolescent mental health mutually influenced each other, and further, warmth was the most influential parenting practice on adolescent mental health. The temporal network revealed that maternal hostility exerted the most influence on adolescent mental health problems, whereas adolescents' depression was most influenced by maternal parenting practices. Moreover, maternal hostility was most predicted by maternal harshness. CONCLUSIONS: This study presents a novel perspective to gain a better understanding of the dynamics between and within maternal parenting practices and adolescent mental health problems. Findings highlight maternal harshness and warmth as potential prevention and intervention targets for adolescent mental health problems.

Impacts of poverty eradication on carbon neutrality in China.

China is facing challenges to tackle the threat of climate change while reducing social inequality. Poverty eradication requires improvement in the living conditions of low-income households, which leads in turn to higher carbon footprints and may undermine the efforts of climate change mitigation. Previous studies have assessed the climate impacts of poverty eradication, but few have quantified how the additional carbon emissions of poverty eradication are shared at the subnational level in China and the impact on China's climate targets. We investigated the recent trend of carbon footprint inequality in China's provinces and estimated the climate burden of different poverty reduction schemes, measured by increased carbon emissions. The results indicate that poverty eradication will not impede the achievement of national climate targets, with an average annual household carbon footprint increase of 0.1%-1.2%. However, the carbon emissions growth in less developed provinces can be 4.0%, five times that in wealthy regions. Less developed regions suffer a greater climate burden because of poverty eradication, which may offset carbon reduction efforts. Therefore, interregional collaboration is needed to coordinate inequality reduction with investments in low-carbon trajectories in all provinces.

Molecular identification and functional characterization of a transcription factor GeRAV1 from Gelsemium elegans.

BACKGROUND: Gelsemium elegans is a traditional Chinese medicinal plant and temperature is one of the key factors affecting its growth. RAV (related to ABI3/VP1) transcription factor plays multiple roles in higher plants, including the regulation of plant growth, development, and stress response. However, RAV transcription factor in G. elegans has not been reported. RESULTS: In this study, three novel GeRAV genes (GeRAV1-GeRAV3) were identified from the transcriptome of G. elegans under low temperature stress. Phylogenetic analysis showed that GeRAV1-GeRAV3 proteins were clustered into groups II, IV, and V, respectively. RNA-sequencing (RNA-seq) and real-time quantitative PCR (qRT-PCR) analyses indicated that the expression of GeRAV1 and GeRAV2 was increased in response to cold stress. Furthermore, the GeRAV1 gene was successfully cloned from G. elegans leaf. It encoded a hydrophilic, unstable, and non-secretory protein that contained both AP2 and B3 domains. The amino acid sequence of GeRAV1 protein shared a high similarity of 81.97% with Camptotheca acuminata CaRAV. Subcellular localization and transcriptional self-activation experiments demonstrated that GeRAV1 was a nucleoprotein without self-activating activity. The GeRAV1 gene was constitutively expressed in the leaves, stems, and roots of the G. elegans, with the highest expression levels in roots. In addition, the expression of the GeRAV1 gene was rapidly up-regulated under abscisic acid (ABA), salicylic acid (SA), and methyl jasmonate (MeJA) stresses, suggesting that it may be involved in hormonal signaling pathways. Moreover, GeRAV1 conferred improved cold and sodium chloride tolerance in Escherichia coli Rosetta cells. CONCLUSIONS: These findings provided a foundation for further understanding on the function and regulatory mechanism of the GeRAV1 gene in response to low-temperature stress in G. elegans.

The global mismatch between equitable carbon dioxide removal liability and capacity.

Limiting climate change to 1.5°C and achieving net-zero emissions would entail substantial carbon dioxide removal (CDR) from the atmosphere by the mid-century, but how much CDR is needed at country level over time is unclear. The purpose of this paper is to provide a detailed description of when and how much CDR is required at country level in order to achieve 1.5°C and how much CDR countries can carry out domestically. We allocate global CDR pathways among 170 countries according to 6 equity principles and assess these allocations with respect to countries' biophysical and geophysical capacity to deploy CDR. Allocating global CDR to countries based on these principles suggests that CDR will, on average, represent ∼4% of nations' total emissions in 2030, rising to ∼17% in 2040. Moreover, equitable allocations of CDR, in many cases, exceed implied land and carbon storage capacities. We estimate ∼15% of countries (25) would have insufficient land to contribute an equitable share of global CDR, and ∼40% of countries (71) would have insufficient geological storage capacity. Unless more diverse CDR technologies are developed, the mismatch between CDR liabilities and land-based CDR capacities will lead to global demand for six GtCO2 carbon credits from 2020 to 2050. This demonstrates an imperative demand for international carbon trading of CDR.

Factors Driving China's Carbon Emissions after the COVID-19 Outbreak.

The outbreak of the coronavirus disease 2019 (COVID-19) may exert profound impacts on China's carbon emissions via structural changes. Due to a lack of data, previous studies have focused on quantifying the changes in carbon emissions but have failed to identify structural changes in the determinants of carbon emissions. Here, we use China's latest input-output table and apply structural decomposition analyses to understand the dynamic changes in the determinants of carbon emissions from 2012 to 2020, specifically the impact of COVID-19 on carbon emissions. We find that final demand per capita contributed to emissions growth at a slower pace, but production structure drove a greater carbon emissions increase than before the pandemic. Export-led emissions growth rebounded, and investment-led emissions were more concentrated in the construction sector. The carbon intensity of several heavy industries increased, e.g., the nonmetallic products sector, the metal products sector, and the petroleum, coking, and nuclear fuel sector. In addition, lower production efficiency and increased reliance on carbon-intensive inputs indicated a deterioration in production structure. For policy implications, efforts should be undertaken to increase investment in low-carbon industries and increase the proportion of consumption in GDP to shift investment-led growth to consumption-led growth for an inclusive and green recovery from the pandemic.

The impact of the COVID-19 pandemic on the behaviour of bike sharing users.

Globally most governments implemented a 'Working from Home' (home office) strategy to contain the spread of the coronavirus in 2020 in order to ensure public safety and minimize the transmission of the virus. Unsurprisingly studies have found that COVID-19 has had a detrimental impact on urban transportation systems; however, the number of shared bicycle riders is progressively growing compared to other modes of public transit. The aim of this study is to investigate the influence of COVID-19 on the usage of shared bicycle systems in order to identify passenger travel patterns and habits. In addition, bicycle rentals are becoming more popular in some locations. This demonstrates that bike sharing as a transport option has a high level of social adaptability and is progressively being adopted by the general population in a fashion that promotes the resilience of transport systems.

Efficiency improvement by navigated safety inspection involving visual clutter based on the random search model.

Navigated inspection seeks to improve hazard identification (HI) accuracy. With a tight inspection schedule, HI also requires efficiency. However, lacking quantification of HI efficiency, navigated inspection strategies cannot be comprehensively assessed. This work aims to determine inspection efficiency in navigated safety inspection, controlling for HI accuracy. Based on a cognitive method of the random search model (RSM), an experiment was conducted to observe the HI efficiency in navigation, for a variety of visual clutter (VC) scenarios, while using eye-tracking devices to record the search process and analyze the search performance. The results show that the RSM is an appropriate instrument, and VC serves as a hazard classifier for navigation inspection in improving inspection efficiency. This suggests a new and effective solution for addressing the low accuracy and efficiency of manual inspection through navigated inspection involving VC and the RSM. It also provides insights into the inspectors' safety inspection ability.

Influence of visual clutter on the effect of navigated safety inspection: a case study on elevator installation.

Introduction. Navigated safety inspection based on task-specific checklists can increase the hazard detection rate, theoretically with interference from scene complexity. Visual clutter, a proxy of scene complexity, can theoretically impair visual search performance, but its impact on the effect of safety inspection performance remains to be explored for the optimization of navigated inspection. This research aims to explore whether the relationship between working memory and hazard detection rate is moderated by visual clutter. Methods. Based on a perceptive model of hazard detection, we: (a) developed a mathematical influence model for construction hazard detection; (b) designed an experiment to observe the performance of hazard detection rate with adjusted working memory under different levels of visual clutter, while using an eye-tracking device to observe participants' visual search processes; (c) utilized logistic regression to analyze the developed model under various visual clutter. Conclusion. The effect of a strengthened working memory on the detection rate through increased search efficiency is more apparent in high visual clutter. This study confirms the role of visual clutter in construction-navigated inspections, thus serving as a foundation for the optimization of inspection planning.

Removal of the tag from His-tagged ILYd4, a human CD59 inhibitor, significantly improves its physical properties and its activity.

Complement dependent cytotoxicity (CDC) significantly contributes to Rituximab (RTX) and Ofatumumab (OFA) efficacies in the treatment of B-cell non-Hodgkin's lymphoma (NHL) and chronic lymphocytic leukemia (CLL). Human CD59 (hCD59) is a key complement regulatory protein that restricts the formation of the membrane attack complex and thereby inhibits CDC. hCD59 is an important determinant of the sensitivity of NHL and CLL to RTX and OFA treatment. Recently, we developed a specific and potent hCD59 inhibitor, His-tagged ILYd4, which consists of 30 amino acid sequences extending from the N-terminus of ILYd4. Our previously published results indicate that His-tagged ILYd4 can be used as a lead candidate to further develop a potential therapeutic adjuvant for RTX and OFA treatment of RTX-resistant NHL and CLL. However, these studies were conducted using ILYd4 tagged on the N-terminus with 30 additional amino acids (AA) containing 6 X His used for immobilized metal affinity chromatograph. As a further step towards the development of ILYd4-based therapeutics, we investigated the impact of the removal of this extraneous sequence on the anti-hCD59 activity. In this paper, we report the generation and characterization of tag-free ILYd4. We demonstrate that tag-free ILYd4 has over threefold higher anti-hCD59 activities than the His-tagged ILYd4. The enhanced RTX-mediated CDC effect on B-cell malignant cells comes from tag-free ILYd4's improved functionality and physical properties including better solubility, reduced tendency to aggregation, and greater thermal stability. Therefore, tag-free ILYd4 is a better candidate for the further development for the clinical application.